LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • EIA Competition Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 11G2) IHC-plus™ LS-B10262

Note: This antibody replaces LS-C114486

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

SERPINA1 / Alpha 1 Antitrypsin Antibody LS-B10262 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver.
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1...
Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal...
Immunoprecipitation
Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative...

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 11G2] (IgG1) to Human SERPINA1 / Alpha 1 Antitrypsin
Human, Monkey, Dog
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human SERPINA1 / Alpha 1 Antitrypsin
Mouse
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [11G2]
Protein A/G purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (1:1000 - 1:2000), Immunoprecipitation

Specificity and Use

SERPINA1 / Alpha 1 Antitrypsin antibody was raised against protein expressed in 293T cell transfected with human SERPINA1 expression vector.
Human SERPINA1 / Alpha 1 Antitrypsin
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SERPINA1 / Alpha 1 Antitrypsin

P01009 K02212 AAB59495.1

SERPINA1 Antibody, A1A Antibody, A1AT Antibody, AAT Antibody, Alpha 1 Antitrypsin Antibody, Alpha-1 protease inhibitor Antibody, Alpha-1-antiproteinase Antibody, Alpha-1-antitrypsin Antibody, Alpha1AT Antibody, PRO2275 Antibody, Alpha-1-antitrypsin null Antibody, PI1 Antibody, Serpin A1 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10262 concentration 10 ug/ml.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Requested From: United States
Date Requested: 8/30/2015

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2015 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number