Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin (1:20 - 1:40)
Specificity and Use
Serotonin antibody was raised against 5-Hydroxytryptamine hydrochloride (3-(2-aminoethyl)-5-hydroxyindole; serotonin).
Reacts with serotonin-containing cells in a wide range of normal, reactive, and neoplastic tissues. Other endocrine cells, such as those of pancreatic islets and pituitary gland, are negative with this antibody. Species cross-reactivity: Human.
Immunohistology: (Formalin/paraffin) (Use Ab at 1:20-1:40 for 30 min at RT) For staining of formalin-fixed tissues, digest sections with Protease XXV at 1 mg/ml PBS for 5-10 minutes at 37?C. Positive control: Appendix.
0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.