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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEPW1 / Selenoprotein W Antibody LS‑C60114
Selenoprotein W antibody LS-C60114 is an unconjugated rabbit polyclonal antibody to mouse Selenoprotein W (SEPW1). Validated for ELISA and WB.
Catalog
Size
Price
LS-C60114-100
100 µg
$425

Popular SEPW1 / Selenoprotein W Products

Immunohistochemistry of paraffin-embedded human heart tissue using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Western blot of recombinant SEPW1 / Selenoprotein W.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Western blot of recombinant SEPW1 / Selenoprotein W.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Western blot of recombinant SEPW1 / Selenoprotein W.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot

Product Description

Selenoprotein W antibody LS-C60114 is an unconjugated rabbit polyclonal antibody to mouse Selenoprotein W (SEPW1). Validated for ELISA and WB.
About SEPW1 / Selenoprotein W
SEPW1 / Selenoprotein W is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. P63302 NM_003009 NP_003000.1

SEPW1 Antibody, Selenoprotein W Antibody, SelW Antibody, Selenoprotein W, 1 Antibody


Specifications

Target
Mouse SEPW1 / Selenoprotein W
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:100000)
Immunogen
Recombinant protein corresponding to full-length mouse SelW protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with SelW from rat based on a 98% homology with the immunizing sequence. Partial reactivity is expected against human-derived SelW based on an 87% homology with the immunogen. Cross-reactivity with SelW from other sources has not been determined
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 9.6 kD in size corresponding to SelW by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both overexpressed and endogenous SelW.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.

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Images

Western blot

Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.

Western blot

Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.

Western blot

Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.

Western blot

Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Anti-SeIW Antibody - Western Blot. Western blot of anti-SelW antibody shows detection of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW protein at 9.6 kD. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.

Requested From: United States
Date Requested: 9/25/2018

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