Human SEPT2 / Septin 2

Goat

Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence SKQQPTQFINPET-C, from the N-Terminus of protein sequence according to NP_001008491.1.

aa37-49

Human SEPT2 / Septin 2. Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (0.05 - 0.2 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Immunohistochemistry: LS-B3607 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3607 was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 40kD band observed in Human, Mouse and Rat Testis lysates (calculated MW of 41.5kD according to NP_001008491.1). Recommended concentration: 0.05-0.2 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15019 NCBI: NM_004404 NP_004395.1