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SEPT12 / Septin 12 Antibody (aa180‑229) LS‑C102452
Septin 12 antibody LS-C102452 is an unconjugated rabbit polyclonal antibody to Septin 12 (SEPT12) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102452-50
50 µg
Unavailable

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Product Description

Septin 12 antibody LS-C102452 is an unconjugated rabbit polyclonal antibody to Septin 12 (SEPT12) from human, mouse, rat and other species. Validated for WB.
About SEPT12 / Septin 12
SEPT12 / Septin 12 is a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. Q8IYM1 Q8IYM1

SEPT12 Antibody, Septin-12 Antibody, Septin 12 Antibody, SPGF10 Antibody


Specifications

Target
Human SEPT12 / Septin 12
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Dog, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa180-229 of human SEPT12 (Q8IYM1, NP_653206). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Gibbon, Dog, Opossum (92%); Platypus (91%); Turkey, Salmon, Stickleback, Zebrafish (90%); Xenopus (84%).
Blocking Peptide
LS-E18980 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102452. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa180-229
Specificity
Human SEPT12 / Septin 12
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SEPT12 / Septin 12 antibody LS-C102452 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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