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Anti-SEPT11 / Septin 11 Antibody (N-Terminus) IHC-plus™ LS-B4024

Note: This antibody replaces LS-C102721

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SEPT11 / Septin 11 Antibodies

Anti-SEPT11 / Septin 11 Antibody (N-Terminus) IHC-plus™ LS-B4024
Rabbit Polyclonal (IgG) to Human SEPT11 / Septin 11
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SEPT11 / Septin 11 Antibody (aa352-382, FITC) LS-C240578
Rabbit Polyclonal (IgG) to Human SEPT11 / Septin 11
Human
Western blot, ELISA
FITC Conjugated
Anti-SEPT11 / Septin 11 Antibody (aa352-382, PE) LS-C240581
Rabbit Polyclonal (IgG) to Human SEPT11 / Septin 11
Human
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SEPT11 / Septin 11
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SEPT11 / Septin 11
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

SEPT11 / Septin 11 antibody was raised against synthetic peptide from N-Terminus of human SEPT11 (Q9NVA2, NP_060713). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Opossum (85%).
LS-E20858 - Lyophilized - 100 µg - $145.00
N-Terminus
Human SEPT11 / Septin 11
Immunohistochemistry: LS-B4024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4024 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SEPT11 / Septin 11

Q9NVA2 NM_018243 NP_060713.1

SEPT11 Antibody, Septin 11 Antibody, Septin-11 Antibody

Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy.

Immunohistochemistry

Immunohistochemistry
Anti-SEPT11 / Septin 11 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4024 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-SEPT11 / Septin 11 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4024 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western analysis of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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