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Anti-SEPHS2 Antibody LS-C59553

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SEPHS2 Antibodies

Anti-SEPHS2 Antibody (Internal, AP) LS-C283301
Rabbit Polyclonal (IgG) to Human SEPHS2
Human
IHC, Western blot, ELISA
AP Conjugated
Anti-SEPHS2 Antibody (Internal, Biotin) LS-C283303
Rabbit Polyclonal (IgG) to Human SEPHS2
Human
IHC, Western blot, ELISA
Biotin Conjugated
Anti-SEPHS2 Antibody (Internal, PE) LS-C283306
Rabbit Polyclonal (IgG) to Human SEPHS2
Human
IHC, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse SEPHS2
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse SEPHS2
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • ELISA (1:5000 - 1:20000)

Specificity and Use

SEPHS2 antibody was raised against full-length recombinant protein corresponding to mouse SPS2.
mouse SPS2 and shows partial cross-reactivity with SPS1. A BLAST analysis was used to suggest cross-reactivity with SPS2 from human sources based on an 84% homology with the immunizing sequence. Cross-reactivity with SPS2 from other sources has not been determined
This Protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kD in size corresponding to SPS2 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About SEPHS2

Q99611 NM_012248 NP_036380.2

SEPHS2 Antibody, Selenophosphate synthase 2 Antibody, SPS2b Antibody, Selenium donor protein 2 Antibody, Selenophosphate synthetase 2 Antibody, SPS2 Antibody, Selenide, water dikinase 2 Antibody

SEPHS2 is an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. Genes encoding selenocysteine contain a stem-loop secondary structure in their 3' UTR called a selenocysteine insertion sequence (SECIS) element. The protein encoded by this gene contains a selenocysteine residue in its predicted active site.

Western blot

Western blot
Anti-SPS2 Antibody - Western Blot. Western blot of Protein A purified anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
 

Requested From: United States
Date Requested: 12/8/2016

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