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Catalog Number Size Price
LS-C497142-20 20 µl $275 
LS-C497142-100 100 µl (6.67 mg/ml) $389 
SEMA4F / Semaphorin 4F Antibody - Western blot analysis of extracts of various cell lines, using SEMA4F antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

Polyclonal Rabbit anti‑Human SEMA4F / Semaphorin 4F Antibody (WB) LS‑C497142

Polyclonal Rabbit anti‑Human SEMA4F / Semaphorin 4F Antibody (WB) LS‑C497142

Antibody:
SEMA4F / Semaphorin 4F Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C497142-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SEMA4F / Semaphorin 4F Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Semaphorin 4F antibody LS-C497142 is an unconjugated rabbit polyclonal antibody to Semaphorin 4F (SEMA4F) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human SEMA4F / Semaphorin 4F
Synonyms
SEMA4F | M-SEMA | S4F | Sema M | Semaphorin M | Semaphorin-4F | Semaphorin-W | SEMAW | PRO2353 | Sema W | SEMAM | M-Sema-M | Semaphorin-M
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 280-420 of human SEMA4F (NP_004254.2). RKTLQQRWTTFLKADLLCPGPEHGRASSVLQDVAVLRPELGAGTPIFYGIFSSQWEGATISAVCAFRPQDIRTVLNGPFRELKHDCNRGLPVVDNDVPQPRPGECITNNMKLRHFGSSLSLPDRVLTFIRDHPLMDRPVFP
Applications
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 66kDa/83kDa, while the observed MW by Western blot was 84kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SEMA4F / Semaphorin 4F
O95754 NM_004263 NP_004254.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024