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Anti-SEMA4C / Semaphorin 4C Antibody (aa21-663) LS-C150056

Ordering

Wt. Vol. Conc. Price
100 µg - 0.2 mg/ml $395
Inquire for larger quantities

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Most Popular SEMA4C / Semaphorin 4C Antibodies

Anti-SEMA4C / Semaphorin 4C Antibody (aa21-663) LS-C150056
Sheep Polyclonal (IgG) to Human SEMA4C / Semaphorin 4C
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SEMA4C / Semaphorin 4C Antibody (aa22-664) LS-C150248
Sheep Polyclonal (IgG) to Mouse SEMA4C / Semaphorin 4C
Mouse
IHC, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SEMA4C / Semaphorin 4C Antibody (aa792-821, APC) LS-C217439
Rabbit Polyclonal (IgG) to Human SEMA4C / Semaphorin 4C
Human
Western blot, Flow Cytometry, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human SEMA4C / Semaphorin 4C
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human SEMA4C / Semaphorin 4C
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.5 µg/ml)

Specificity and Use

SEMA4C / Semaphorin 4C antibody was raised against mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C Ala21-Gly663 Accession # Q9C0C4.
aa21-663
Detects human Semaphorin 4C in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant.

Packaging

Lyophilized from 0.2 um sterile-filtered solution in PBS, 5% trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SEMA4C / Semaphorin 4C

Q9C0C4 NM_017789 NP_060259.4

SEMA4C Antibody, M-Sema F Antibody, SEMAF Antibody, SEMACL1 Antibody, Semaphorin-4C Antibody, KIAA1739 Antibody, M-SEMA-F Antibody, SEMAI Antibody

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration By similarity. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.

Immunohistochemistry

Immunohistochemistry
Semaphorin 4C in Human Brain. Semaphorin 4C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies.

Western blot

Western blot
Detection of Human Semaphorin 4C by Western Blot. Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 0.5 ug/ml of Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Semaphorin 4C at approximately 110 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/3/2016

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