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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEMA4A / Semaphorin 4A Antibody (aa501‑550) LS‑C119263 Best Seller
Semaphorin 4A antibody LS-C119263 is an unconjugated rabbit polyclonal antibody to Semaphorin 4A (SEMA4A) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C119263-50
50 µl
Unavailable
LS-C119263-100
100 µl
Unavailable

Popular SEMA4A / Semaphorin 4A Products

IHC of SEMA4A (N532) pAb in paraffin-embedded human brain tissue.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: Flow Cytometry
Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Monkey
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot of SEMA4A antibody
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

Semaphorin 4A antibody LS-C119263 is an unconjugated rabbit polyclonal antibody to Semaphorin 4A (SEMA4A) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About SEMA4A / Semaphorin 4A
SEMA4A / Semaphorin 4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Q9H3S1 NM_022367 NP_071762.2

SEMA4A Antibody, CORD10 Antibody, RP11-54H19.2 Antibody, RP35 Antibody, Sema B Antibody, Semaphorin-4A Antibody, Semaphorin-B Antibody, SEMB Antibody, SEMAB Antibody


Specifications

Target
Human SEMA4A / Semaphorin 4A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human SEMA4A (aa501-550).
Epitope
aa501-550
Specificity
SEMA4A Antibody detects endogenous levels of total SEMA4A protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/18/2018

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