Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human SEMA3E / Semaphorin 3E
Mouse Monoclonal (IgG1) to Human SEMA3E / Semaphorin 3E
Western blot, ELISA
Human SEMA3E / Semaphorin 3E
Human (tested or 100% immunogen sequence identity)
Protein G purified
Western blot (1 - 2 µg/ml)
ELISA (0.5 - 1 µg/ml)
Specificity and Use
NS0-derived rhSemaphorin 3E.
Recognizes human Semaphorin 3E in direct ELISAs and Western Blots. In Western Blots, this antibody shows less than 10% cross-reactivity with rhSemaphorin 3A, -3B, -6A, -6B, -6C, -6D, -7A, rmSemaphorin 3C, -3E, -3F.
Suitable for use in ELISA and Western Blot. ELISA: 0.5-1 ug/ml Detection limit is ~13 ng/well. Western Blot: 1-2 ug/ml Detection limit is ~10 ng/lane under non-reducing and reducing conditions.
Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis.