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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEMA3E / Semaphorin 3E Antibody LS‑C39037
Antibody
Semaphorin 3E antibody LS-C39037 is an unconjugated goat polyclonal antibody to human Semaphorin 3E (SEMA3E). Validated for ELISA, ICC, IHC and WB.
Catalog
Size
Price
LS-C39037-100
100 µg (0.2 mg/ml)
Unavailable

Popular SEMA3E / Semaphorin 3E Products

Antibody
Species: Human
Applications: Western blot, ELISA
Western blot analysis of Semaphorin 3E expression in A549 (A); mouse liver (B); rat liver (C) whole cell lysates.
Species: Human, Mouse, Rat
Applications: Western blot
Western blot of SEMA3E / Semaphorin 3E antibody.
Species: Human
Applications: IHC, Western blot
Antibody
Species: Human
Applications: Western blot

Product Description

Semaphorin 3E antibody LS-C39037 is an unconjugated goat polyclonal antibody to human Semaphorin 3E (SEMA3E). Validated for ELISA, ICC, IHC and WB.
About SEMA3E / Semaphorin 3E
Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. O15041 AB002329 NP_036563.1

SEMA3E Antibody, M-SEMAH Antibody, SEMAH Antibody, Semaphorin 3E Antibody, M-SemaK Antibody, Semaphorin-3E Antibody, Coll-5 Antibody, KIAA0331 Antibody


Specifications

Target
Human SEMA3E / Semaphorin 3E
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (10 µg/ml)
  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NS0-derived rhSemaphorin 3E.
Specificity
Recognizes human Semaphorin 3E.
Usage
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect Semaphorin 3E on MDA-MB-453 human breast cancer cells. Immunohistochemistry: 10 ug/ml to detect Semaphorin 3E in cryosections of E13.5 mouse embryos. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 1.0 ng/well. In this format, this antibody shows approximately 70% cross-reactivity with rmSemaphorin 3E and less than 5% cross-reactivity with rmSemaphorin 3C and rmSemaphorin 3F.
Presentation
Lyophilized from PBS, 5% trehalose.
Reconstitution
500 µl Reconstitute with sterile 40-50% glycerol, PBS.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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