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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEMA3E / Semaphorin 3E Antibody LS‑C39037
Semaphorin 3E antibody LS-C39037 is an unconjugated goat polyclonal antibody to human Semaphorin 3E (SEMA3E). Validated for ELISA, ICC, IHC and WB.
Catalog
Size
Price
LS-C39037-100
100 µg (0.2 mg/ml)
$770
Description
Semaphorin 3E antibody LS-C39037 is an unconjugated goat polyclonal antibody to human Semaphorin 3E (SEMA3E). Validated for ELISA, ICC, IHC and WB.
Target
Human SEMA3E / Semaphorin 3E
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (10 µg/ml)
  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NS0-derived rhSemaphorin 3E.
Specificity
Recognizes human Semaphorin 3E.
Usage
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect Semaphorin 3E on MDA-MB-453 human breast cancer cells. Immunohistochemistry: 10 ug/ml to detect Semaphorin 3E in cryosections of E13.5 mouse embryos. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 1.0 ng/well. In this format, this antibody shows approximately 70% cross-reactivity with rmSemaphorin 3E and less than 5% cross-reactivity with rmSemaphorin 3C and rmSemaphorin 3F.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
500 µl Reconstitute with sterile 40-50% glycerol, PBS.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SEMA3E / Semaphorin 3E
O15041 AB002329 NP_036563.1

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Requested From: United States
Date Requested: 12/10/2018
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