Human SEMA3E / Semaphorin 3E
(tested or 100% immunogen sequence identity)
- IHC (10 µg/ml)
- ICC (10 µg/ml)
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
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NS0-derived rhSemaphorin 3E.
Recognizes human Semaphorin 3E.
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect Semaphorin 3E on MDA-MB-453 human breast cancer cells. Immunohistochemistry: 10 ug/ml to detect Semaphorin 3E in cryosections of E13.5 mouse embryos. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 1.0 ng/well. In this format, this antibody shows approximately 70% cross-reactivity with rmSemaphorin 3E and less than 5% cross-reactivity with rmSemaphorin 3C and rmSemaphorin 3F.
Lyophilized from PBS, 5% Trehalose
500 µl Reconstitute with sterile 40-50% glycerol, PBS.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.