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Antibodies > SELP / P-Selectin / CD62P > LS-C357551
 
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Catalog Number Size Price
LS-C357551-10 10 µg $318 
LS-C357551-100 100 µg (0.5 mg/ml) $470 
SELP / P-Selectin / CD62P Antibody - IHC analysis of CD62P using anti-CD62P antibody. CD62P was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD62P Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SELP / P-Selectin / CD62P Antibody - ABCG2 antibody, PB9364, IHC-paraffin: Human Lung Cancer Tissue.
SELP / P-Selectin / CD62P Antibody - Western blot analysis of CD62P using anti-CD62P antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: K562 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD62P at approximately 91KD. The expected band size for CD62P is at 140KD.
SELP / P-Selectin / CD62P Antibody - ABCG2 antibody, PB9364, Western blot. All lanes: Anti ABCG2 (PB9364) at 0.5 ug/ml. Lane 1: Human Placenta Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 72 kD. Observed band size: 72 kD.
SELP / P-Selectin / CD62P Antibody - Flow Cytometry analysis of THP-1 cells using anti-P-Selectin antibody. Overlay histogram showing THP-1 cells stained with anti-P-Selectin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P-Selectin Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
SELP / P-Selectin / CD62P Antibody - IHC analysis of CD62P using anti-CD62P antibody. CD62P was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD62P Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SELP / P-Selectin / CD62P Antibody - ABCG2 antibody, PB9364, IHC-paraffin: Human Lung Cancer Tissue.
SELP / P-Selectin / CD62P Antibody - Western blot analysis of CD62P using anti-CD62P antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: K562 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD62P at approximately 91KD. The expected band size for CD62P is at 140KD.
SELP / P-Selectin / CD62P Antibody - ABCG2 antibody, PB9364, Western blot. All lanes: Anti ABCG2 (PB9364) at 0.5 ug/ml. Lane 1: Human Placenta Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 72 kD. Observed band size: 72 kD.
SELP / P-Selectin / CD62P Antibody - Flow Cytometry analysis of THP-1 cells using anti-P-Selectin antibody. Overlay histogram showing THP-1 cells stained with anti-P-Selectin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P-Selectin Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human SELP / P‑Selectin / CD62P Antibody (aa42‑271, IHC, WB) LS‑C357551

Polyclonal Rabbit anti‑Human SELP / P‑Selectin / CD62P Antibody (aa42‑271, IHC, WB) LS‑C357551

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Note: This antibody replaces LS-C389024
Antibody:
SELP / P-Selectin / CD62P Rabbit anti-Human Polyclonal (aa42-271) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357551-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SELP / P-Selectin / CD62P Rabbit anti-Human Polyclonal (aa42-271) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD62P antibody LS-C357551 is an unconjugated rabbit polyclonal antibody to human CD62P (SELP / P-Selectin) (aa42-271). Validated for ELISA, IHC and WB.
Target
Human SELP / P-Selectin / CD62P
Synonyms
SELP | CD62P | CD62 | GMP-140 | GMRP | Granule membrane protein 140 | LECAM3 | P-selectin | PSEL | CD62P antigen | GMP140 | Granulocyte membrane protein | GRMP | PADGEM
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CD62P recombinant protein (Position: W42-G271). Human CD62P shares 75.2% and 75.7% amino acid (aa) sequence identity with mouse and rat CD62P, respectively.
Epitope
aa42-271
Specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SELP / P-Selectin / CD62P
NM_003005 NP_002996.2

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Species: Human
Applications: IHC, IHC - Paraffin, Immunoprecipitation, Flow Cytometry
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SELP / P-Selectin / CD62P Antibody - Flow cytometry of thrombin activated human peripheral blood platelets with Mouse anti-Human CD62P:RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.
SELP / P‑Selectin / CD62P Antibody (clone Psel.KO.2.5, RPE) LS‑C188305
Species: Human
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SELP / P-Selectin / CD62P Antibody - Western Blot; Sample: Recombinant SELP, Human.
SELP / P‑Selectin / CD62P Antibody (aa58‑195) LS‑C296359
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Applications: Western blot
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Species: Human
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SELP / P-Selectin / CD62P Antibody - Western Blot; Sample: Recombinant protein.
SELP / P‑Selectin / CD62P Antibody (aa58‑158, Biotin) LS‑C297504
Species: Rat
Applications: Western blot
See all SELP / P-Selectin / CD62P Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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