Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone MEL-14] (IgG2a,k) to Mouse SELL / L-Selectin / CD62L
Immunoprecipitation, Flow Cytometry
Mouse SELL / L-Selectin / CD62L
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [MEL-14]
Specificity and Use
SELL / L-Selectin / CD62L antibody was raised against mouse SELL
The MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
SELL / L-Selectin / CD62L is a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites.
Staining of BALB/c splenocytes with 0.25 ug of Purified Rat IgG2a isotype control (open histogram) or 0.25 ug of Purified anti-mouse CD62L (MEL-14) (colored histogram) followed by FITC Anti-Rat IgG. Cells in the lymphocyte gate were used for analysis.
Requested From: United States
Date Requested: 1/23/2017