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Anti-SELE / CD62E / E-selectin Antibody LS-C13897

Ordering

Wt. Vol. Conc. Price
100 µg - - $605
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SELE / CD62E / E-selectin Antibodies

Anti-4-Hydroxynonenal Antibody LS-C56815
Goat Polyclonal
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human SELE / CD62E / E-selectin
Human, Monkey
IHC - Frozen, Immunoprecipitation
Unconjugated

Details

Human SELE / CD62E / E-selectin
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Purified

Applications

IHC - Frozen, Immunoprecipitation

Specificity and Use

Reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). Reacts with human endothelial cells activated with TNF-a, IL-1 or endotoxin. Found to react also with cells transfected with the E-selectin gene. Inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
The antibody is useful for staining of E-selectin expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. The antibody can also be used for staining of chimpanzee, rhesus monkey, cynomolgus monkey and baboon endothelial cells expressing E-selectin. Furthermore the antibody can be used for Immunoprecipitation and immunoassays. Immunohistology: 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-a (1 ng/ml), IL-1 (100U/ml) or LPS (1 ug/ml) in tissue culture medium at 37 C. As negative control it is advised to use a control murine IgG1 antibody.

Packaging

PBS, 0.02% sodium azide, 0.1% BSA.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About SELE / CD62E / E-selectin

P16581 NM_000450 NP_000441.2

SELE Antibody, CD62E Antibody, ELAM Antibody, ESEL Antibody, E-selectin Antibody, LECAM2 Antibody, CD62E antigen Antibody, ELAM-1 Antibody, ELAM1 Antibody, Selectin E Antibody

SELE / CD62E / E-selectin is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules.

Requested From: United States
Date Requested: 10/1/2016

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