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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Securin / PTTG1 Antibody (N‑Terminus) LS‑C9840
PTTG1 antibody LS-C9840 is an unconjugated rabbit polyclonal antibody to human PTTG1 (Securin). Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C9840-100
100 µg (0.25 mg/ml)
$605

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Product Description

PTTG1 antibody LS-C9840 is an unconjugated rabbit polyclonal antibody to human PTTG1 (Securin). Validated for ELISA, IHC and WB.
About Securin / PTTG1
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. O95997 NM_004219 NP_004210.1

PTTG1 Antibody, EAP1 Antibody, Esp1-associated protein Antibody, HPTTG Antibody, ESP1-associated protein 1 Antibody, Pituitary tumor-transforming 1 Antibody, TUTR1 Antibody, PTTG Antibody, Securin Antibody, Tumor-transforming protein 1 Antibody


Specifications

Target
Human Securin / PTTG1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1 - 2 µg/ml)
  • Western blot (2 - 4 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Securin / PTTG1 antibody was raised against synthetic peptide derived from the transactivation domain within the N-terminus of the human PTTG-1 protein.
Epitope
N-Terminus
Specificity
Recognizes human PTTG-1. Expected to cross-react with mouse PTTG-1 based sequence homology. Reactivity confirmed with human MCF-7 cell lysate (Western Blot) and colon cancer tissue (IHC, paraffin).
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 2-4 ug/ml. Recognizes only the 28kD band in MCF-7 cell lysate. Immunohistochemistry: 1-2 ug/ml in formalin-fixed, paraffin embedded tissues. For best results heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/20/2018

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