Human Securin / PTTG1
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin (1 - 2 µg/ml)
- Western blot (2 - 4 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Securin / PTTG1 antibody was raised against synthetic peptide derived from the transactivation domain within the N-terminus of the human PTTG-1 protein.
Recognizes human PTTG-1. Expected to cross-react with mouse PTTG-1 based sequence homology. Reactivity confirmed with human MCF-7 cell lysate (Western Blot) and colon cancer tissue (IHC, paraffin).
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 2-4 ug/ml. Recognizes only the 28kD band in MCF-7 cell lysate. Immunohistochemistry: 1-2 ug/ml in formalin-fixed, paraffin embedded tissues. For best results heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.