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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SCYL3 Antibody (clone 2H10) LS‑C114591
SCYL3 antibody LS-C114591 is an unconjugated mouse monoclonal antibody to human SCYL3. Validated for IHC and WB.
Catalog
Size
Price
LS-C114591-100
100 µl
Unavailable

Popular SCYL3 Products

IHC of paraffin-embedded Carcinoma of kidney tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded pancreas tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IHC of paraffin-embedded H1299 xenograft using SCY1 like 3 antibody at 1:500 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Antibody
Species: Mouse
Applications: IHC, Western blot, ELISA

Product Description

SCYL3 antibody LS-C114591 is an unconjugated mouse monoclonal antibody to human SCYL3. Validated for IHC and WB.
About SCYL3
SCYL3 is a protein with a kinase domain and four HEAT repeats. The encoded protein interacts with the C-terminal domain of ezrin, an ERM protein, and may play a role in cell adhesion and migration. Alternative splicing results in multiple transcript variants encoding multiple isoforms. Q8IZE3 NM_020423 NP_065156.5

SCYL3 Antibody, Ezrin-binding protein PACE-1 Antibody, PACE-1 Antibody, SCY1-like 3 (S. cerevisiae) Antibody, PACE1 Antibody, SCY1-like protein 3 Antibody


Specifications

Target
Human SCYL3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2H10]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SCYL3 antibody was raised against protein expressed in 293T cell transfected with human SCYL3 expression vector.
Specificity
Human SCYL3
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.


Requested From: 
Date Requested: 6/19/2018

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