(tested or 100% immunogen sequence identity)
SCARF1 / SREC antibody was raised against nS0-derived recombinant human SREC-I.
Direct ELISA: 0.5-1.0ug/ml with the appropriate secondaryreagents to detect human SREC-I. The detection limit for rhSREC-Iis approximately 1ng/well and 100ng/ml under non-reducingand reducing conditons, respectively.Flow Cytometry: 0.25ug/ml. Use 10ul of the diluted antibody tostain 1x10e5 to 2.5 x 10e5 cells in a total reaction volume notexceeding 200ul. A fluorochrome-labeled secondary antibodysuch a goat anti-mouse IgG may be used for detection.Western Blot: 1-2ug/ml with the appropriate secondary reagentsto detect human SREC-1. Using a colorimetric detection system,the detection limit for rhSREC-1 is approximately 1ng/lane and100ng/lane under non-reducing and reducing conditions,respectively. Chemiluminescent detection will increase sensitivityby 5 to 50 fold.Optimal dilutions should be determined by each laboratory foreach application.
Supplied as a powder lyophilized from a solution containing PBS with 5% trehalose.