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Antibodies > SCAP > LS-B9086
 
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Catalog Number Size Price
LS-B9086-200 200 µl (0.48 mg/ml) $460 
SCAP Antibody - Anti-SCAP antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
SCAP Antibody - Fluorescent confocal image of HeLa cells stained with SCAP antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SCAP primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
SCAP Antibody - Western blot analysis of Anti-SCAP antibody (LS-B9086, 1:2000 dilution; 20 µg of lysate/protein per lane). Lane 1: HeLa whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: LNCap whole cell lysate. Lane 5: Mouse brain lysate. Lane 6: Mouse liver lysate. Lane 7: Rat heart lysate. Antibody produced band at ~95 kDa
SCAP Antibody - Western blot analysis of Anti-SCAP antibody (LS-B9086; 35 µg per lane). Lane 1: K562 whole cell lysate. Antibody produced band at ~95 kDa
SCAP Antibody - Flow cytometric of K562 cells using SCAP Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SCAP Antibody - Anti-SCAP antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
SCAP Antibody - Fluorescent confocal image of HeLa cells stained with SCAP antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SCAP primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
SCAP Antibody - Western blot analysis of Anti-SCAP antibody (LS-B9086, 1:2000 dilution; 20 µg of lysate/protein per lane). Lane 1: HeLa whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: LNCap whole cell lysate. Lane 5: Mouse brain lysate. Lane 6: Mouse liver lysate. Lane 7: Rat heart lysate. Antibody produced band at ~95 kDa
SCAP Antibody - Western blot analysis of Anti-SCAP antibody (LS-B9086; 35 µg per lane). Lane 1: K562 whole cell lysate. Antibody produced band at ~95 kDa
SCAP Antibody - Flow cytometric of K562 cells using SCAP Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human SCAP Antibody (aa604‑632, IHC, IF, WB) LS‑B9086

IHC‑plus™ Polyclonal Rabbit anti‑Human SCAP Antibody (aa604‑632, IHC, IF, WB) LS‑B9086

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C162886
Antibody:
SCAP Rabbit anti-Human Polyclonal (aa604-632) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9086-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SCAP Rabbit anti-Human Polyclonal (aa604-632) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SCAP antibody LS-B9086 is an unconjugated rabbit polyclonal antibody to SCAP (aa604-632) from human. It is reactive with human, mouse and rat. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SCAP
Synonyms
SCAP | KIAA0199 | SREBF chaperone
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa604-632
Specificity
This SCAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-632 amino acids from the Central region of human SCAP present in isoforms 1, 2, and 4.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:25)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SCAP
Q12770 D83782 BAA12111.2

Validation

SCAP Antibody - Anti-SCAP antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SCAP antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SCAP antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ SCAP Antibody (aa604-632) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B9086 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

Submit a Publication for LS-B9086

Customer Reviews (0)

Submit a review for LS-B9086

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PTN / Pleiotrophin ELISA Kit
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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