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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SATB1 Antibody (C‑Terminus) LS‑C115970
SATB1 antibody LS-C115970 is an unconjugated rabbit polyclonal antibody to SATB1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C115970-50
50 µg
Unavailable

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Product Description

SATB1 antibody LS-C115970 is an unconjugated rabbit polyclonal antibody to SATB1 from human, mouse, rat and other species. Validated for WB.
About SATB1
Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Q01826 NM_002971 NP_002962.1

SATB1 Antibody, DNA-binding protein SATB1 Antibody, SATB homeobox 1 Antibody


Specifications

Target
Human SATB1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Guinea pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
SATB1 antibody was raised against synthetic peptide from C-Terminus of human SATB1 (Q01826, NP_002962). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Pig, Opossum, Platypus (100%); Monkey, Bat, Guinea pig (92%); Lizard (85%); Turkey, Zebra finch, Chicken (84%).
Blocking Peptide
LS-E11772 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C115970. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human SATB1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SATB1 antibody LS-C115970 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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