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Anti-SARS NSP3 Antibody (aa834-943) LS-C59283

Ordering

Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SARS NSP3 Antibodies

Anti-SARS NSP3 Antibody (aa834-943) LS-C59283
Rabbit Polyclonal to SARS Coronavirus SARS NSP3
SARS Coronavirus
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunofluorescence Image

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Rabbit Polyclonal to SARS Coronavirus SARS NSP3
SARS Coronavirus
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

SARS Coronavirus SARS NSP3
Rabbit
SARS Coronavirus (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • Immunofluorescence (1:1000)
  • Western blot (1:1000)
  • Immunoprecipitation

Specificity and Use

SARS NSP3 antibody was raised against with 3 E. coli derived proteins representing different parts of the SARS-CoV Nsp3 protein: aa 834-943 of polyprotein 1a (ADRP domain), aa 1208-1557 of polyprotein 1a (SARS-Unique Domain) and aa 1558-2040 of polyprotein 1a (Papain-like proteinase domain).
aa834-943
SARS-Coronavirus nsp3 protein. Cross reactivity with homologs from other sources has not been determined
This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 211 kD in size corresponding to SARS-CoV nsp3 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:500;

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About SARS NSP3


Immunofluorescence

Immunofluorescence
Anti-SARS-CoV nsp3 Protein Antibody - Immunofluorescence Microscopy. Immunofluorescence Microscopy using anti-SARS-CoV nsp3 protein antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX ( was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
 

Requested From: United States
Date Requested: 12/3/2016

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