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Anti-SARS NSP13 Antibody (C-Terminus) LS-C59285

Ordering

Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular SARS NSP13 Antibodies

Anti-SARS NSP13 Antibody (C-Terminus) LS-C59285
Rabbit Polyclonal to SARS Coronavirus SARS NSP13
SARS Coronavirus
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to SARS Coronavirus SARS NSP13
SARS Coronavirus
Immunofluorescence, Western blot
Unconjugated

Details

SARS Coronavirus SARS NSP13
Rabbit
SARS Coronavirus (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • Immunofluorescence (1:800)
  • Western blot

Specificity and Use

SARS NSP13 antibody was raised against bSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 584-601) of the SARS Coronavirus nonstructural protein 13.
C-Terminus
SARS-Coronavirus nsp13 protein. Cross reactivity with homologs from other sources has not been determined
This antibody has been tested for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kD in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About SARS NSP13


Immunofluorescence

Immunofluorescence
Anti-SARS-CoV nsp13 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using anti-SARS-CoV nsp13 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX ( was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
 

Requested From: United States
Date Requested: 12/7/2016

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