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Anti-SARS / Serine-tRNA Ligase Antibody (clone 1H4) LS-C133424

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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866-206-6909
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Most Popular SARS / Serine-tRNA Ligase Antibodies

Anti-SARS / Serine-tRNA Ligase Antibody (aa241-290) LS-C31216
Rabbit Polyclonal (IgG) to Human SARS / Serine-tRNA Ligase
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Horse, Rabbit, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-SARS / Serine-tRNA Ligase Antibody (C-Terminus) LS-C31217
Rabbit Polyclonal (IgG) to Human SARS / Serine-tRNA Ligase
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-SARS / Serine-tRNA Ligase Antibody LS-C334914
Rabbit Polyclonal (IgG) to Human SARS / Serine-tRNA Ligase
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1H4] (IgG2a,k) to Human SARS / Serine-tRNA Ligase
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human SARS / Serine-tRNA Ligase
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1H4]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (3 µg/ml)
  • Immunofluorescence (25 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

SARS / Serine-tRNA Ligase antibody was raised against sARS (AAH00716, 1 a.a. ~ 515 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human Seryl-tRNA synthetase
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About SARS / Serine-tRNA Ligase

P49591 NM_006513 NP_006504.2

SARS Antibody, SERRS Antibody, SERS Antibody, Seryl-tRNA Ser/Sec synthetase Antibody, Seryl-tRNA synthetase Antibody, Seryl-tRNA(Ser/Sec) synthetase Antibody

Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).

Immunohistochemistry

Immunohistochemistry
Immunoperoxidase of monoclonal antibody to SARS on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml].

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to SARS on HeLa cell. [antibody concentration 25 ug/ml]

Western blot

Western blot
SARS monoclonal antibody (M01), clone 1H4 Western blot of SARS expression in Jurkat.

Western blot

Western blot
Western blot of SARS expression in transfected 293T cell line by SARS monoclonal antibody (M01), clone 1H4.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged SARS is approximately 0.03 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of SARS over-expressed 293 cell line, cotransfected with SARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SARS monoclonal antibody, clone 1H4. GAPDH ( 36.1 kD ) used as specificity an.

Requested From: United States
Date Requested: 12/8/2016

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