Coronavirus SARS-CoV SARS-CoV 3CL Protease

Rabbit

Coronavirus SARS-CoV, Virus (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease.  Lifesensors Inc. (www.lifesensors.com) prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography.

• Immunofluorescence
• Western blot (1:2000 - 1:10000)
• ELISA (1:10000 - 1:50000)

This protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kD in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.