Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to SARS Coronavirus SARS 3CL Protease
Western blot, ELISA
SARS Coronavirus SARS 3CL Protease
SARS Coronavirus (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:2000 - 1:10000)
ELISA (1:10000 - 1:50000)
Specificity and Use
SARS Coronavirus 3CL Protease. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. Cross reactivity with homologs from other sources has not been determined
This protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kD in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
100 µl Reconstitute with 100ul deionized water
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Anti-SARS CoV 3CL Protease Antibody - Western Blot. Western blot of Protein A Purified anti-SARS CoV 3CL Protease antibody shows detection of a 34-kD band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5000 dilution of the antibody at room temperature for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:10000. The fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.