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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SARA2 / SAR1B Antibody (aa104‑116) LS‑C55434
SAR1B antibody LS-C55434 is an unconjugated goat polyclonal antibody to SAR1B (SARA2) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55434-100
100 µg
Unavailable

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Product Description

SAR1B antibody LS-C55434 is an unconjugated goat polyclonal antibody to SAR1B (SARA2) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
About SARA2 / SAR1B
SARA2 / SAR1B is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. Q9Y6B6 NM_016103 NP_057187.1

SAR1B Antibody, ANDD Antibody, CMRD Antibody, GTP-binding protein SAR1b Antibody, GTP-binding protein Sara Antibody, SARB Antibody, SAR1a gene homolog 2 Antibody, SARA2 Antibody, GTBPB Antibody, GTP-binding protein B Antibody, SAR1 homolog B (S. cerevisiae) Antibody


Specifications

Target
Human SARA2 / SAR1B
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:8000) & (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Immunogen
SARA2 / SAR1B antibody was raised against synthetic peptide C-DHERLLESKEELD from an internal region of human SAR1B (NP_001028675.1; NP_057187.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%); Turkey, Chicken (92%); Platypus (85%).
Blocking Peptide
LS-E26394 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55434. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa104-116
Specificity
Human SAR1B. Both reported variants (NP_001028675.1 and NP_057187.1) represent identical protein
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).
HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).

Western blot

Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).
HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).

Western blot

Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).
HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).

Western blot

Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).
HEK293 overexpressing SAR1B (RC210593) and probed with (mock transfection in first lane).

Western blot

Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/18/2018

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