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Anti-SAMSN1 Antibody (aa218-267) LS-C80783

Catalog	Size	Price
LS-C80783-50	50 µg	Unavailable

Related Products

22 SAMSN1 Antibodies

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Anti-SAMSN1 Antibody (C-Terminus) LS-C153726

Antibody:	Rabbit Polyclonal to Human SAMSN1
Reactivity:	Human
Application:	Western blot
Format:	Unconjugated

Anti-SAMSN1 Antibody (aa255-283) LS-C162862

Antibody:	Rabbit Polyclonal to Human SAMSN1
Reactivity:	Human
Application:	IHC - Paraffin, Western blot, Flow Cytometry
Format:	Unconjugated

Anti-SAMSN1 Antibody (aa255-283, AP) LS-C268765

Antibody:	Rabbit Polyclonal (IgG) to Human SAMSN1
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	AP Conjugated

Anti-SAMSN1 Antibody (aa255-283, APC) LS-C268766

Antibody:	Rabbit Polyclonal (IgG) to Human SAMSN1
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	APC Conjugated

Anti-SAMSN1 Antibody (aa262-291) LS-C324907

Antibody:	Rabbit Polyclonal (IgG) to Human SAMSN1
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	Unconjugated

Antibody:	Rabbit Polyclonal (IgG) to Human SAMSN1
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human SAMSN1
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Guinea pig (at least 90% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

SAMSN1 antibody was raised against synthetic peptide located between aa218-267 of human SAMSN1 (Q9NSI8, NP_071419). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Pig (100%); Gorilla, Guinea pig, Platypus (92%); Bat, Horse, Opossum, Chicken (85%).

Blocking Peptide:

LS-E16737 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80783. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa218-267

Specificity:

Human SAMSN1

Usage:

ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	50 µl Distilled Water.
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About SAMSN1

Uniprot: Q9NSI8 NCBI: NM_022136 NP_071419.3

Synonyms: SAMSN1 Antibody, SH3-SAM adaptor protein Antibody, NASH1 Antibody, SASH2 Antibody, SLy2 Antibody, HACS1 Antibody, SH3D6B Antibody

Description: Negative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton. Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation.

(More About SAMSN1)

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Technical Help Review

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Western blot

Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 8/23/2017

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