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Anti-SAE1 Antibody (phospho-Ser185) LS-C154247


Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular SAE1 Antibodies

Anti-SAE1 Antibody IHC-plus™ LS-B420
Rabbit Polyclonal to Human SAE1
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-SAE1 Antibody (C-Terminus) LS-C108720
Rabbit Polyclonal to Human SAE1
Human, Mouse, Rat
Western blot, ELISA
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SAE1
Human, Mouse, Rat, Bovine, Dog
Western blot, ELISA


Human SAE1
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
IgG Polyclonal
Affinity purified


  • Western blot (2 µg/ml)

Specificity and Use

SAE1 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein.
This antibody is specific for human SAE1 protein phosphorylated at S185. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kD in size corresponding to phosphorylated SAE1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific antibody reacts with human SAE1 pS185 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About SAE1

Q9UBE0 NM_005500 NP_005491.1

SAE1 Antibody, Activator of SUMO1 Antibody, AOS1 Antibody, UBLE1A Antibody, HSPC140 Antibody, SUA1 Antibody

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Western blot

Western blot
Anti-SAE1 pS185 Antibody - Western Blot. Western blot of Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX.

Requested From: United States
Date Requested: 10/26/2016

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