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Anti-SAE1 Antibody (aa334-346) LS-C55427

Catalog Size Price
LS-C55427-100 100 µg Unavailable

Related Products

46 SAE1 Antibodies

Most Popular SAE1 Antibodies

Anti-SAE1 Antibody IHC-plus™ LS-B420
Rabbit Polyclonal to Human SAE1
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B420 concentration 5 ug/ml.
Anti-SAE1 Antibody (clone 1G4-1G5) IHC-plus™ LS-B6203
Mouse Monoclonal [clone 1G4-1G5] (IgG1,l) to Human SAE1
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-SAE1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6203 concentration 3 ug/ml.
Anti-SAE1 Antibody (clone 1G8) IHC-plus™ LS-B9408
Mouse Monoclonal [clone 1G8] (IgG2b) to Human SAE1
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Anti-SAE1 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SAE1 Antibody (Internal) LS-C353229
Rabbit Polyclonal to Human SAE1
Human, Monkey
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of SAE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-SAE1 Antibody (aa190-270) LS-C385989
Rabbit Polyclonal (IgG) to Human SAE1
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of SAE1 antibody

100% Guaranteed
Goat Polyclonal to Human SAE1
Human, Monkey, Bat, Horse, Rabbit
Western blot, ELISA
Unconjugated

Details

Human SAE1
Goat
Human, Monkey, Bat, Horse, Rabbit (tested or 100% immunogen sequence identity)
Bovine, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:8000) & (1 µg/ml)
  • ELISA (1:8000)

  • IHC - Paraffin

Specificity and Use

SAE1 antibody was raised against synthetic peptide MKGNGIVECLGPK from the C-terminus of human SAE1 (NP_005491.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Bat, Horse, Rabbit (100%); Panda, Bovine, Pig, Opossum (92%); Mouse, Rat, Elephant (85%).
LS-E25600 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55427. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa334-346
Human SAE1.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About SAE1

Q9UBE0 NM_005500 NP_005491.1

SAE1 Antibody, Activator of SUMO1 Antibody, AOS1 Antibody, UBLE1A Antibody, HSPC140 Antibody, SUA1 Antibody

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Western blot

Staining (1?g/ml) of Jurkat lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1?g/ml) of Jurkat lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: 
Date Requested: 4/22/2018

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