Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 47-8D3] (IgG1) to Human S100A9 / MRP14
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Human S100A9 / MRP14
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [47-8D3]
Protein G purified
IHC - Paraffin (1:100)
IHC - Frozen
Specificity and Use
S100A9 / MRP14 antibody was raised against human peripheral blood monocyte components. Splenocytes were fused with mouse myeloma cell line NS-1.
Reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosisantigen. Using Western blot analysis 47-8D3 was found to detect a single protein band of 14 kDin lysates of human monocytes and granulocytes, which was demonstrated to be the calcium-bindingprotein MRP14.
Staining is strongly enhanced after digestion with trypsin.
PBS with 0.05% Sodium Azide. Sourced in TCS.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions.