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Anti-S100A8 / MRP8 Antibody (FITC) IHC-plus™ LS-B2247

Note: This antibody replaces LS-C25355

Ordering

Wt. Vol. Conc. Price
50 µg - 0.1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular S100A8 / MRP8 Antibodies

Anti-S100A8 / MRP8 Antibody (clone CF-145) IHC-plus™ LS-B1618
Mouse Monoclonal [clone CF-145] (IgG2b) to Human S100A8 / MRP8
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-S100A8 / MRP8 Antibody (FITC) IHC-plus™ LS-B2247
Mouse Monoclonal (IgG1) to Human S100A8 / MRP8
Human, Monkey, Rat, Bovine, Cat, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Flow Cytometry
FITC Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human S100A8 / MRP8
Human, Monkey, Rat, Bovine, Cat, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Flow Cytometry
FITC Conjugated

Details

Human S100A8 / MRP8
Mouse
Human, Monkey, Rat, Bovine, Cat, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Fluorescein
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Flow Cytometry

Specificity and Use

S100A8 / MRP8 antibody was raised against human monocytes.
Recognizes human L1 Protein or Calprotectin Molecule. Variable results have been reported for the staining of brain macrophages and microglia. Species cross-reactivity: equine, Cynomolgus monkey, Rhesus monkey, monkey, bovine, baboon, rabbit, canine, feline, porcine, guinea pig and rat.
Immunohistochemistry: LS-B2247 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2247 was determined to be 1:50. Flow Cytometry: Neat. Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 1% BSA, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About S100A8 / MRP8

P05109 NM_002964 NP_002955.2

S100A8 Antibody, 60B8AG Antibody, CFAG Antibody, CAGA Antibody, Calgranulin A Antibody, Calgranulin-A Antibody, Calprotectin L1L subunit Antibody, Cystic fibrosis antigen Antibody, CGLA Antibody, L1Ag Antibody, NIF Antibody, p8 Antibody, Protein S100-A8 Antibody, MRP8 Antibody, Urinary stone protein band A Antibody, CP-10 Antibody, MA387 Antibody, MRP-8 Antibody

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions.

Immunohistochemistry

Immunohistochemistry
Anti-S100A8 antibody IHC of human spleen, granulocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2247 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/6/2016

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