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Anti-S100A8 / MRP8 Antibody (aa1-93) LS-C150054

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular S100A8 / MRP8 Antibodies

Anti-S100A8 / MRP8 Antibody (clone CF-145) IHC-plus™ LS-B1618
Mouse Monoclonal [clone CF-145] (IgG2b) to Human S100A8 / MRP8
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-S100A8 / MRP8 Antibody (FITC) IHC-plus™ LS-B2247
Mouse Monoclonal (IgG1) to Human S100A8 / MRP8
Human, Monkey, Rat, Bovine, Cat, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Flow Cytometry
FITC Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human S100A8 / MRP8
Human
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human S100A8 / MRP8
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

S100A8 / MRP8 antibody was raised against e. coli-derived recombinant human S100A8 Met1-Glu93 Accession # P05109.
aa1-93
Detects human S1 00A8 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant mouse S100A8.
Western Blot, 1 µg/ml. Immunocytochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About S100A8 / MRP8

P05109 NM_002964 NP_002955.2

S100A8 Antibody, 60B8AG Antibody, CFAG Antibody, CAGA Antibody, Calgranulin A Antibody, Calgranulin-A Antibody, Calprotectin L1L subunit Antibody, Cystic fibrosis antigen Antibody, CGLA Antibody, L1Ag Antibody, NIF Antibody, p8 Antibody, Protein S100-A8 Antibody, MRP8 Antibody, Urinary stone protein band A Antibody, CP-10 Antibody, MA387 Antibody, MRP-8 Antibody

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions.

Immunocytochemistry

Immunocytochemistry
S1 00A8 in HeLa Human Cell Line. S100A8 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human S1 00A8 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Western blot

Western blot
Detection of Human S100A8 by Western Blot. Western blot shows lysates of human brain tissue, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Human S1 00A8 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for S1 00A8 at approximately 10 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of S1 00A8 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by NorthernLights 637-conjugated Anti-Sheep IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
 

Requested From: United States
Date Requested: 12/5/2016

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