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Anti-S100A10 Antibody IHC-plus™ LS-B3445

Note: This antibody replaces LS-C41471

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular S100A10 Antibodies

Anti-S100A10 Antibody IHC-plus™ LS-B3445
Mouse Monoclonal (IgG1) to Human S100A10
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-S100A10 Antibody (aa12-40) LS-C162827
Rabbit Polyclonal to Human S100A10
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-S100A10 Antibody (aa3-52) IHC-plus™ LS-B12569
Rabbit Polyclonal (IgG) to Human S100A10
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human S100A10
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human S100A10
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot
  • ELISA (1:10000)

Specificity and Use

S100A10 antibody was raised against recombinant Human S100A10.
Ni-NTA purified truncated recombinant P11 expressed in E. Coli strain BL21 (DE3)
Immunohistochemistry: LS-B3445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3445 was determined to be 1:200.

Packaging

PBS, pH 7.4, 0.03% sodium azide.
For research use only.

About S100A10

P60903 NM_002966 NP_002957.1

S100A10 Antibody, 42C Antibody, ANX2LG Antibody, Calpactin-1 light chain Antibody, Calpactin I light chain Antibody, CLP11 Antibody, p11 Antibody, p10 Antibody, p10 protein Antibody, Protein S100-A10 Antibody, ANX2L Antibody, Ca[1] Antibody, CAL1L Antibody, Cellular ligand of annexin II Antibody, gp11 Antibody

S100A10 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.

Immunohistochemistry

Immunohistochemistry
Anti-S100A10 antibody IHC of human nerve and ganglion cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3445 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-S100A10 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3445 dilution 1:200.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of HeLa (left) and L-02 (right) cells using S100A10/P11 mouse monoclonal antibody(green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot
Western blot using S100A10/P11 mouse monoclonal antibody against MCF-7 (1), HepG2 (2) and HeLa (3).

Requested From: United States
Date Requested: 12/10/2016

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