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Anti-RYK Antibody (aa160-190) LS-C99131

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RYK Antibodies

Anti-RYK Antibody (aa287-533, FITC) LS-C302113
Rabbit Polyclonal (IgG) to Mouse RYK
Mouse
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human RYK
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human RYK
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

Specificity and Use

RYK antibody was raised against kLH-conjugated synthetic peptide between amino acids 160-190 from human RYK.
LS-E9183 - Lyophilized - 100 µg 1 mg/ml - $145.00
aa160-190

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About RYK

P34925 NM_002958 NP_002949.2

RYK Antibody, D3S3195 Antibody, Hydroxyaryl-protein kinase Antibody, JTK5 Antibody, JTK5A protein tyrosine kinase Antibody, RYK1 Antibody, Vik Antibody, Tyrosine-protein kinase RYK Antibody, Receptor-like tyrosine kinase Antibody, JTK5A Antibody, Tyrosine kinase ryk Antibody

RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RYK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
Western blot of RYK (arrow) using rabbit polyclonal RYK Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).

Western blot

Western blot
Western blot of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
The anti-RYK antibody is used in Western blot to detect RYK in Jurkat cell lysate.

Western blot

Western blot
Western blot of lysate from human ovary tissue lysate, using RYK Antibody (N175). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
Western blot of lysate from human ovary tissue lysate, using RYK Antibody (N175). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
Western blot of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Requested From: United States
Date Requested: 12/10/2016

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