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Anti-RUNX1T1 / ETO Antibody (clone 5A12) LS-C196824

Catalog Size Price
LS-C196824-100 100 µg Unavailable

Related Products

24 RUNX1T1 / ETO Antibodies

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Anti-RUNX1T1 / ETO Antibody (aa221-270) LS-C31287
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Western blot
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HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RUNX1T1 / ETO Antibody (aa288-337) LS-C112160
Rabbit Polyclonal to Human RUNX1T1 / ETO
Human, Monkey, Rat, Dog, Pig, Rabbit
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RUNX1T1 antibody LS-C112160 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RUNX1T1 / ETO Antibody (clone 5A12) LS-C196824
Mouse Monoclonal [clone 5A12] (IgG2a,k) to Human RUNX1T1 / ETO
Human
Immunofluorescence, Western blot, ELISA
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Immunofluorescence of monoclonal antibody to RUNX1T1 on HeLa cell . [antibody concentration 10 ug/ml]
Anti-RUNX1T1 / ETO Antibody (aa554-604) IHC-plus™ LS-B13526
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IHC, IHC - Paraffin, Western blot, Immunoprecipitation
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Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-RUNX1T1 / ETO Antibody LS-C403498
Rabbit Polyclonal (IgG) to Human RUNX1T1 / ETO
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IHC, ELISA
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Immunohistochemistry of Human thyroid cancer using RUNX1T1 Polyclonal Antibody at dilution of 1:35.

100% Guaranteed
Mouse Monoclonal [clone 5A12] (IgG2a,k) to Human RUNX1T1 / ETO
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human RUNX1T1 / ETO
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [5A12]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

RUNX1T1 / ETO antibody was raised against rUNX1T1 (NP_004340, aa416-525 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human RUNX1T1 / ETO
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About RUNX1T1 / ETO

Q06455 NM_004349 NP_004340.1

RUNX1T1 Antibody, AML1T1 Antibody, CBFA2T1 Antibody, CDR Antibody, Eight twenty one protein Antibody, Cyclin-D-related protein Antibody, ETO Antibody, MTG8 Antibody, Protein CBFA2T1 Antibody, Protein ETO Antibody, ZMYND2 Antibody, Protein MTG8 Antibody

RUNX1T1 / ETO is a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene.

Immunofluorescence

Immunofluorescence of monoclonal antibody to RUNX1T1 on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to RUNX1T1 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RUNX1T1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RUNX1T1 is approximately 0.3 ng/ml as a capture antibody.

Requested From: 
Date Requested: 5/22/2018

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