Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
RTN4 / Nogo antibody was raised against an 18aa peptide in the C-terminal, cytoplasmic domain of rat Nogo-A (KLH coupled).
Recognizes Neurite Outgrowth Inhibitor Protein A (NogoA). NogoA11 epitope is not present in alternatively spliced shorter forms of Nogo-B and Nogo-C. Species sequence Homology: mouse: 94%; human: 83%. No significant sequence homology is detected with Rtn family members.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. The calculated mol wt of Nogo-A is ~135kD. However, a MW of ~180kD has been reported for full length Nogo-A. ELISA: 1:10K-1:100K using 50-100 ng of control peptide/well.
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity).