Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100)
Western blot (1:500 - 1:2000)
Specificity and Use
RTN4 / Nogo antibody was raised against a synthetic sequence corresponding to amino acids 14-30 (DSPPRPQPAFKYQFVRE) of human Nogo-B was used as the immunogen, GenBank no. AAG40878.1|AF320999_1. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Pig (100%); Bovine (94%); Mouse, Rat, Bat, Dog (88%); Opossum (82%).
This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
This antibody recognizes Nogo-A (approximately 220 kD)and Nogo-B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
PBS and 0.05% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity).