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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RTKN / Rhotekin Antibody (aa107‑156) LS‑C111839
Rhotekin antibody LS-C111839 is an unconjugated rabbit polyclonal antibody to Rhotekin (RTKN) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C111839-50
50 µg
Unavailable

Popular RTKN / Rhotekin Products

Anti-RTKN antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4410 concentration 10 ug/ml.
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IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RTKN mouse monoclonal antibody.
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Immunohistochemistry of paraffin-embedded human kidney tissue.
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IHC of paraffin-embedded Human endometrium tissue using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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Applications: IHC, Western blot, ELISA

Product Description

Rhotekin antibody LS-C111839 is an unconjugated rabbit polyclonal antibody to Rhotekin (RTKN) from human, mouse, rat and other species. Validated for WB.
About RTKN / Rhotekin
RTKN / Rhotekin is a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Q9BST9 AF290512 AAG01181.1

RTKN Antibody, Rhotekin Antibody, RTKN1 Antibody


Specifications

Target
Human RTKN / Rhotekin
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RTKN / Rhotekin antibody was raised against synthetic peptide located between aa107-156 of human RTKN (Q9BST9, NP_001015055). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Stickleback (92%); Xenopus (85%).
Blocking Peptide
LS-E20366 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111839. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa107-156
Specificity
Human RTKN
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RTKN antibody LS-C111839 Western blot of Fetal Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTKN antibody LS-C111839 Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RTKN antibody LS-C111839 Western blot of Fetal Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTKN antibody LS-C111839 Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RTKN antibody LS-C111839 Western blot of Fetal Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTKN antibody LS-C111839 Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RTKN antibody LS-C111839 Western blot of Fetal Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTKN antibody LS-C111839 Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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