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RTCD1 / RPC Antibody (aa36‑85) LS‑C101847
RPC antibody LS-C101847 is an unconjugated rabbit polyclonal antibody to RPC (RTCD1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C101847-50
50 µg
Unavailable

Popular RTCD1 / RPC Products

Western blot of extracts from Jurkat cells, using RTCD1 Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Immunohistochemical analysis of RPC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

RPC antibody LS-C101847 is an unconjugated rabbit polyclonal antibody to RPC (RTCD1) from human, mouse, rat and other species. Validated for WB.
About RTCD1 / RPC
Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. O00442 NM_003729 NP_003720.1

RTCA Antibody, RNA cyclase Antibody, RNA-3-phosphate cyclase Antibody, RTCD1 Antibody, RTC1 Antibody, RPC Antibody, RPC1 Antibody, RTC domain containing 1 Antibody


Specifications

Target
Human RTCD1 / RPC
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RTCD1 / RPC antibody was raised against synthetic peptide located between aa36-85 of human RTCA (O00442, NP_003720). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus, Salmon, Stickleback, Zebrafish (100%); Zebra finch, Chicken, Lizard (92%).
Blocking Peptide
LS-E14775 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101847. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa36-85
Specificity
Human RTCD1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RTCD1 antibody LS-C101847 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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