Mouse RSPO3

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

400404

Unconjugated

Protein G purified

Unmodified

E. coli-derived rmRSPO-3.

Selected for its ability to detect recombinant mouse RSPO-3 in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rmRSPO-1, rmRSPO-4, rhRSPO-2, or rhRSPO-3.

• Western blot
• ELISA

Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect mouse RSPO-3. Using a colorimetric detection system, the detection limit for rmRSPO-3 is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse RSPO-3. The detection limit for rmRSPO-3 is approximately 1 ng/well.

Lyophilized from PBS, 5% Trehalose

Reconstitute in sterile PBS.

Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9BXY4 NCBI: NM_032784 NP_116173.2