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Anti-RSPO3 Antibody (aa181-193) LS-C139710

Catalog Size Price
LS-C139710-100 100 µg (0.5 mg/ml) Unavailable

Related Products

31 RSPO3 Antibodies

Most Popular RSPO3 Antibodies

Anti-RSPO3 Antibody (clone 400404) LS-C38971
Rat Monoclonal [clone 400404] (IgG2a) to Mouse RSPO3
Mouse
Western blot, ELISA
Unconjugated
Anti-RSPO3 Antibody (aa181-193) LS-C139710
Goat Polyclonal to Human RSPO3
Human, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
RSPO3 antibody (0.3 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-RSPO3 Antibody (C-Terminus) LS-C336277
Rabbit Polyclonal (IgG) to Human RSPO3
Human, Mouse
ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunocytochemistry of RSPO3 in 293 cells with RSPO3 antibody at 5 µg/mL.
Anti-RSPO3 Antibody (aa22-272, clone 3A9) LS-C339260
Mouse Monoclonal [clone 3A9] (IgG1) to Human RSPO3
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-RSPO3 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Anti-RSPO3 Antibody LS-C380096
Rabbit Polyclonal (IgG) to Human RSPO3
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry of paraffin-embedded human testis using antibody at dilution of 1:100.

100% Guaranteed
Goat Polyclonal to Human RSPO3
Human, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human RSPO3
Goat
Human, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)

Specificity and Use

RSPO3 antibody was raised against synthetic peptide QHPSAKGNLCPPT from an internal region of human RSPO3 (NP_116173.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%); Gibbon, Marmoset, Opossum (92%).
LS-E27534 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139710. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa181-193
Human RSPO3.
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 28kD band observed in Human Skeletal Muscle lysates (calculated MW of 30.9kD according to NP_116173.2). Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About RSPO3

Q9BXY4 NM_032784 NP_116173.2

RSPO3 Antibody, CRISTIN1 Antibody, HRspo3 Antibody, HPWTSR Antibody, R-spondin 3 Antibody, Protein with TSP type-1 repeat Antibody, R-spondin 3 homolog Antibody, THSD2 Antibody, PWTSR Antibody, R-spondin-3 Antibody, Roof plate-specific spondin-3 Antibody

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes.

Western blot

RSPO3 antibody (0.3 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
RSPO3 antibody (0.3 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 5/27/2018

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