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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RSPO1 / RSPO Antibody (N‑Terminus) IHC‑plus™ LS‑B4605 Best Seller
Note: This antibody replaces LS-C108519
RSPO antibody LS-B4605 is an unconjugated rabbit polyclonal antibody to RSPO (RSPO1) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4605-50
50 µg (1 mg/ml)
$425
Description
RSPO antibody LS-B4605 is an unconjugated rabbit polyclonal antibody to RSPO (RSPO1) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RSPO1 / RSPO
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30705 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B4605. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.
Usage
RSPO1 antibody can be used for detection of RSPO1 by Western blot at 1-2 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
About RSPO1 / RSPO
Q2MKA7 Q2MKA7

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IHC of paraffin-embedded Human lymph node tissue using anti-RSPO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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Images

Immunohistochemistry

Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.
Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody  Red: Phylloidin staining Blue: DAPI staining
Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody Red: Phylloidin staining Blue: DAPI staining

Western blot

Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.
Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody  Red: Phylloidin staining Blue: DAPI staining
Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody Red: Phylloidin staining Blue: DAPI staining

Western blot

Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.
Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody  Red: Phylloidin staining Blue: DAPI staining
Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody Red: Phylloidin staining Blue: DAPI staining

Western blot

Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RSPO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.
Immunofluorescence of RSPO1 in Human Spleen cells with RSPO1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody  Red: Phylloidin staining Blue: DAPI staining
Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 µg/ml.Green: RSPO1 antibody Red: Phylloidin staining Blue: DAPI staining

Western blot

Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.

Requested From: United States
Date Requested: 11/17/2018
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