(tested or 100% immunogen sequence identity)
- Western blot (1:500 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:2500 - 1:10000)
RSL1D1 antibody was raised against synthetic peptide corresponding to an internal sequence of human PBK1.
A BLAST analysis was used to suggest limited cross-reactivity with PBK1 from mouse based on a 75% homology with the immunizing sequence. Cross-reactivity with PBK1 from other sources has not been determined.
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75 kD in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Short term: -20°C; Long term: -20°C.
For research use only.