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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RSL1D1 Antibody (Internal) LS‑C60113 Best Seller
RSL1D1 antibody LS-C60113 is an unconjugated rabbit polyclonal antibody to human RSL1D1. Validated for ELISA, IF, IP and WB.
Catalog
Size
Price
LS-C60113-100
100 µg
$425

Popular RSL1D1 Products

RSL1D1 antibody (1 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
RSL1D1 antibody LS-C145829 Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot
RSL1D1 antibody LS-C145829 Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan
Applications: Western blot
RSL1D1 antibody LS-C145829 Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan
Applications: Western blot

Product Description

RSL1D1 antibody LS-C60113 is an unconjugated rabbit polyclonal antibody to human RSL1D1. Validated for ELISA, IF, IP and WB.
O76021 AK000822 O76021

RSL1D1 Antibody, CATX-11 Antibody, CATX11 Antibody, DKFZP564M182 Antibody, L12 Antibody, UTP30 Antibody, PBK1 Antibody, Protein PBK1 Antibody, CSIG Antibody


Specifications

Target
Human RSL1D1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:2500 - 1:10000)
Immunogen
RSL1D1 antibody was raised against synthetic peptide corresponding to an internal sequence of human PBK1.
Epitope
Internal
Specificity
A BLAST analysis was used to suggest limited cross-reactivity with PBK1 from mouse based on a 75% homology with the immunizing sequence. Cross-reactivity with PBK1 from other sources has not been determined.
Usage
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75 kD in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.

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Images

Immunofluorescence

Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Western blot

Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Immunofluorescence

Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Western blot

Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Immunofluorescence

Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Western blot

Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Immunofluorescence

Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Western blot

Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Anti-PBK1 Antibody - Western Blot. Western blot of affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kD, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.

Requested From: United States
Date Requested: 9/23/2018

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