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Anti-RRM2B / P53R2 Antibody (aa2-17) IHC-plus™ LS-B545

Note: This antibody replaces LS-C392, LS-C91193
Catalog Size Price
LS-B545-50 50 µg (0.5 mg/ml) Unavailable

Related Products

27 RRM2B / P53R2 Antibodies

Most Popular RRM2B / P53R2 Antibodies

Anti-RRM2B / P53R2 Antibody (aa2-17) IHC-plus™ LS-B545
Rabbit Polyclonal (IgG) to Human RRM2B / P53R2
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-RRM2B / p53R2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B545 concentration 5 ug/ml.
Anti-RRM2B / P53R2 Antibody (N-Terminus) LS-C154254
Rabbit Polyclonal (IgG) to Human RRM2B / P53R2
Human
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-RRM2B antibody - Western Blot. Western blot of affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4:293T transfected with ScRNA. Lane 5:293T transfected with p53R2 SiRNA The band at 40.7 kD, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kD. The highest band at 44.3 kD corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4C. ECL detection was used.
Anti-RRM2B / P53R2 Antibody (aa318-332) LS-C313179
Rabbit Polyclonal to Human RRM2B / P53R2
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
Western blot
Unconjugated
WB of RRM2B antibody. Lane 1: Rat Thymus Tissue Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: A431 Cell Lysate. Lane 4: HELA Cell Lysate..
Anti-RRM2B / P53R2 Antibody (aa1-351) LS-C377857
Rabbit Polyclonal (IgG) to Human RRM2B / P53R2
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.
Anti-RRM2B / P53R2 Antibody LS-C489005
Rabbit Polyclonal to Human RRM2B / P53R2
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal (IgG) to Human RRM2B / P53R2
Human
IHC, IHC - Paraffin, Western blot
Unconjugated

Details

Human RRM2B / P53R2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)

Specificity and Use

LS-E29954 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B545. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa2-17
At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform.
Immunohistochemistry: LS-B545 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B545 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
For research use only.

About RRM2B / P53R2

Q7LG56 NM_015713 NP_056528.2

RRM2B Antibody, MTDPS8A Antibody, MTDPS8B Antibody, p53R2 Antibody, PEOA5 Antibody

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and g-irradiation that cause DNA damages.

Immunohistochemistry

Anti-RRM2B / p53R2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B545 concentration 5 ug/ml.
Anti-RRM2B / p53R2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B545 concentration 5 ug/ml.

Immunohistochemistry

Anti-RRM2B / p53R2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B545 concentration 5 ug/ml.
Anti-RRM2B / p53R2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B545 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 µg/mL.
Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of p53R2 in Human Lung cells with p53R2 antibody at 20 ug/ml.
Immunofluorescence of p53R2 in Human Lung cells with p53R2 antibody at 20 ug/ml.

Western blot

Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Western blot

Western blot of p53R2 expression in A431 cell lysate at (A) 0.5 and (B) 1 ug /ml.
Western blot of p53R2 expression in A431 cell lysate at (A) 0.5 and (B) 1 ug /ml.

Requested From: 
Date Requested: 5/21/2018

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