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Anti-RRAD Antibody (aa36-48) LS-C154980

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml Unavailable

Related Products

16 RRAD Antibodies

Most Popular RRAD Antibodies

Anti-RRAD Antibody (aa41-90) LS-C120027
Rabbit Polyclonal (IgG) to Human RRAD
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RRAD Antibody (Leu80) LS-C177379
Rabbit Polyclonal to Human RRAD
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RRAD Antibody (aa107-156) IHC-plus™ LS-B11529
Rabbit Polyclonal (IgG) to Human RRAD
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RRAD Antibody (Internal) LS-C352807
Rabbit Polyclonal to Human RRAD
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-RRAD Antibody (aa10-90) LS-C385724
Rabbit Polyclonal (IgG) to Human RRAD
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human RRAD
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Western blot, ELISA
Unconjugated

Details

Human RRAD
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Guinea pig, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.5 - 2 µg/ml)
  • ELISA (1:64000)

Specificity and Use

RRAD antibody was raised against synthetic peptide C-HRRSMPVDERDLQ from an internal region of human RRAD (NP_004156.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Porcine, Opossum (100%); Gorilla, Guinea pig, Chicken (92%); Lizard, Newt, Stickleback, Medaka, Pufferfish (85%).
LS-E27851 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154980. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa36-48
Human RRAD. Reported variants represent identical protein: NP_004156.1, NP_001122322.1
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 37kD band observed in Mouse Heart lysates and no 37kD in the KO lysates (calculated MW of 33.2kD according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged), a band of approx. 45kD is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA. Recommended concentration: 0.5-2 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About RRAD

P55042 NM_004165 NP_004156.1

RRAD Antibody, GTP-binding protein RAD Antibody, Ras associated with diabetes Antibody, REM3 Antibody, RAD Antibody

May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane By similarity. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.

Western blot

Western blot
RRAD antibody a): (0.5 ug/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) and compared with an HA-specific antibody. B): (0.5 ug/ml) staining of WT and KO lysates of Mouse Heart (100 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 4/24/2017

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