Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > RPS6KB1 / P70S6K / S6K > LS-C354087
 
Print Page  
Catalog Number Size Price
LS-C354087-100 100 µl (1 mg/ml) $315 
RPS6KB1 / P70S6K / S6K Antibody - Immunohistochemical analysis of S6K1 (pS447) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
RPS6KB1 / P70S6K / S6K Antibody - Immunofluorescent analysis of S6K1 (pS447) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
RPS6KB1 / P70S6K / S6K Antibody - Western blot analysis of S6K1 (pS447) expression in A549 EGF-treated (A); mouse brain (B); rat brain (C) whole cell lysates.
RPS6KB1 / P70S6K / S6K Antibody - Immunohistochemical analysis of S6K1 (pS447) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
RPS6KB1 / P70S6K / S6K Antibody - Immunofluorescent analysis of S6K1 (pS447) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
RPS6KB1 / P70S6K / S6K Antibody - Western blot analysis of S6K1 (pS447) expression in A549 EGF-treated (A); mouse brain (B); rat brain (C) whole cell lysates.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human RPS6KB1 / P70S6K / S6K Antibody (phospho‑Ser447, IHC, IF, WB) LS‑C354087

Polyclonal Rabbit anti‑Human RPS6KB1 / P70S6K / S6K Antibody (phospho‑Ser447, IHC, IF, WB) LS‑C354087

Submit Review LSBio Guarantee
Antibody:
RPS6KB1 / P70S6K / S6K Rabbit anti-Human Polyclonal (pSer447) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C354087-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RPS6KB1 / P70S6K / S6K Rabbit anti-Human Polyclonal (pSer447) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
S6K antibody LS-C354087 is an unconjugated rabbit polyclonal antibody to S6K (RPS6KB1 / P70S6K) (pSer447) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human RPS6KB1 / P70S6K / S6K
Synonyms
RPS6KB1 | 70 kDa S6 kinase | p70 S6KA | p70-alpha | p70-S6K | p70 S6 kinase alpha | p70s6 kinase | S6K-beta-1 | STK14A | p70 ribosomal S6 kinase | p70 S6 kinase, alpha 1 | p70 S6 kinase, alpha 2 | p70 S6K-alpha | p70-S6K 1 | p70S6K | p70S6K1 | PS6K | Ribosomal protein S6 kinase I | S6K | S6K1 | p70 ribosomal S6 kinase alpha | p70(S6K)-alpha
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human S6K1 (pS447).
Epitope
pSer447
Specificity
Recognizes endogenous levels of S6K1 (pS447) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPS6KB1 / P70S6K / S6K
P23443 NM_003161 NP_003152.1

Publications (0)

Submit a Publication for LS-C354087

Customer Reviews (0)

Submit a review for LS-C354087

Featured Products

Product
RPS6KB1 / P70S6K / S6K Antibody (phospho‑Thr398) LS‑C25949
Species: Drosophila, Human
Applications: Western blot
Product
RPS6KB1 / P70S6K / S6K Antibody (phospho‑Thr389) LS‑C25944
Species: Human
Applications: Western blot, ELISA
RPS6KB1 / P70S6K / S6K Antibody - IHC of paraffin-embedded muscle tissues using RPS6KB1 mouse monoclonal antibody with DAB staining.
RPS6KB1 / P70S6K / S6K Antibody (clone 5G9) LS‑C169467
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
RPS6KB1 / P70S6K / S6K Antibody - Immunohistochemical analysis of S6K1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
RPS6KB1 / P70S6K / S6K Antibody (Internal) LS‑C368704
Species: Human, Mouse, Rat, Bovine, Rabbit
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
RPS6KB1 / P70S6K / S6K Antibody - Anti-RPS6KB1 / S6K antibody IHC of human brain, cortex, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ RPS6KB1 / P70S6K / S6K Antibody (aa337‑386) LS‑B7583
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
RPS6KB1 / P70S6K / S6K Antibody - Anti-RPS6KB1 / S6K antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ RPS6KB1 / P70S6K / S6K Antibody LS‑B419
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
See all RPS6KB1 / P70S6K / S6K Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/15/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com