Rat RPS6KB1 / P70S6K / S6K

Rabbit

Rat, Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

Synthetic peptide corresponding to residues 26-43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.

aa26-43

Recognizes p70 S6 kinase Mr ~64kD. In some cell lysates, an additional protein may be detected at a higher molecular weight. Cross-reacts with rat, human and mouse.

• Western blot
• Immunoprecipitation
• Functional Assay

Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 0.5-2.0 ug/ml detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitates p70 S6 kinase from 500 ug of HEK293 RIPA lysate. Immunoprecipitation Kinase Assay: 5 ug immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1 uM microcystin. The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P23443 NCBI: NM_003161 NP_003152.1