Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Specificity and Use
RPS6KA1 / RSK1 antibody was raised against synthetic phosphopeptide derived from a region of human RSK1 that contains serine 380.
Cross-reaction with RSK2 (85% homologous) is expected based on peptide competition by the analogous sequence from RSK2. Cross-reaction with RSK3 (77% homologous) and RSK4 (85% homologous) is possible but has not been tested. Species cross-reactivity: Cros
Suitable for use in Western Blot. Positive control: A431 and NIH 3T3 cells treated with PMA, a phorbol ester activator of protein kinase C.
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1.