Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Chicken (at least 90% immunogen sequence identity)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
RPS6KA1 / RSK1 antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 303 and 527 (Q15418) of RSK1. Percent identity by BLAST analysis: Human (100%); Bovine (98%); Mouse (97%); Rat (95%); Chicken (91%).
Human RPS6KA1 / RSK1
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1.
RSK1 antibody detects RSK1 p90 protein at Cytoplasm and Nucleus by immunofluorescent analysis. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. RSK1 p90 protein stained by RSK1 antibody diluted at 1:500.
Sample (30 ug whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. RPS6KA1 / RSK1 antibody diluted at 1:1000
Requested From: United States
Date Requested: 2/25/2017