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Anti-RPA70 / RPA1 Antibody (aa323-337) LS-C205059

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RPA70 / RPA1 Antibodies

Anti-RPA70 / RPA1 Antibody (clone 2H10 or 70C or alphaSSB70C, Azide-free) LS-C88376
Mouse Monoclonal [clone 2H10 or 70C or alphaSSB70C] (IgG1,k) to Human RPA70 / RPA1
Human
Western blot, Immunoprecipitation, Gel shift
Unconjugated, Azide-free
Anti-RPA70 / RPA1 Antibody (aa369-616) LS-C186023
Rabbit Polyclonal (IgG) to Human RPA70 / RPA1
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human RPA70 / RPA1
Human, Monkey
Western blot, ELISA
Unconjugated

Details

Human RPA70 / RPA1
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Rat, Guinea pig, Horse, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:128000)

Specificity and Use

RPA70 / RPA1 antibody was raised against synthetic peptide CKSYEDATKITVRSN from an internal region of human RPA70 / RPA1 (NP_002936.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Rat, Ferret, Panda, Rabbit, Horse, Guinea pig (93%); Bat, Elephant (87%); Mouse, Sheep, Chicken, Platypus (80%).
aa323-337
Human RPA70 / RPA1
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70+80kD band observed in lysates of cell line HeLa (calculated MW of 68.1kD according to NP_002936.1). The upper band likely represents the acetylated protein. Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About RPA70 / RPA1

P27694 NM_002945 NP_002936.1

RPA1 Antibody, HSSB Antibody, MST075 Antibody, Replication factor A protein 1 Antibody, Replication protein A1 (70kD) Antibody, RF-A Antibody, RF-A protein 1 Antibody, MSTP075 Antibody, Replication protein A1, 70kDa Antibody, RP-A Antibody, REPA1 Antibody, RP-A p70 Antibody, RPA70 Antibody

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682).

Western blot

Western blot
RPA1 antibody (0.3 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/7/2016

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