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RPA70 / RPA1 Antibody (aa215‑264) LS‑C108039
RPA1 antibody LS-C108039 is an unconjugated rabbit polyclonal antibody to RPA1 (RPA70) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C108039-50
50 µg
Unavailable

Popular RPA70 / RPA1 Products

Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, Gel shift
IHC of paraffin-embedded colon cancer tissues using RPA1 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
IHC of paraffin-embedded Cal27 xenograft using RPA 70 kDa subunit antibody at 1:100 dilution.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Western blot
Detection of Human and Mouse RPA70 by Immunohistochemistry. Sample: FFPE sections of human stomach carcinoma (left) and mouse CT26 colon carcinoma (right). Antibody: Affinity purified rabbit anti-RPA70 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Guinea pig, Pig, Xenopus
Applications: IHC, IHC - Paraffin, Western blot

Product Description

RPA1 antibody LS-C108039 is an unconjugated rabbit polyclonal antibody to RPA1 (RPA70) from human, mouse, rat and other species. Validated for WB.
About RPA70 / RPA1
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). P27694 NM_002945 NP_002936.1

RPA1 Antibody, HSSB Antibody, MST075 Antibody, Replication factor A protein 1 Antibody, Replication protein A1 (70kD) Antibody, RF-A Antibody, RF-A protein 1 Antibody, MSTP075 Antibody, Replication protein A1, 70kDa Antibody, RP-A Antibody, REPA1 Antibody, RP-A p70 Antibody, RPA70 Antibody


Specifications

Target
Human RPA70 / RPA1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Sheep, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Monkey, Guinea pig, Chicken, Zebrafish, Drosophila (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RPA70 / RPA1 antibody was raised against synthetic peptide located between aa215-264 of human RPA1 (P27694, NP_002936). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Xenopus (100%); Marmoset, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Drosophila (92%); Zebrafish (91%); Salmon (90%).
Blocking Peptide
LS-E20538 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C108039. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa215-264
Specificity
Human RPA1 / RPA70
Usage
Western Blot: Suggested dilution at 1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPA1 / RPA70 antibody LS-C108039 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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